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I have been doing PLS analysis on NIR spectroscopy of apple and egg. In both cases absorbance are used in the Y-axis and Wavelength in X-axis. In NIR diagram of raw data, What are absorbance, reflectance? When should each of them be used? What is the physical meaning of absorbance for apple sweetnes…

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I am using UNSCRAMBLER X for soil spectroscopy. I was predicting some values with PLSR, but when I looked at the predicted vs. reference statistics, it showed me “NA” for the R-squared value. I was trying to find out why it doesn’t show zero or the actual value, but didn’t fi…

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