Extrapolating Standard Addition with PLS-RReport
In a univariate experiment one can add known amounts of an analyte to a sample. The equation of the linear regression line y=ax+b can then be extrapolated to where y=0. The concentration of the analyte is then equal to the absolute value of x.
I like to use this method with near-infrared spectrometrie (NIRS) initialy with only 1 analyte. It is relatively straight forward to use PLS-R for standard addition. But how would I calculate the initial analyte concentration?
Kind regards, Kees